Dhananjay Gotarkar

6 Questions 7 Answers 0 Followers

Questions related from Dhananjay Gotarkar

Does anyone have experience of seed germination in Hygromycin solution??

Hi, I want to germinate transgenic rice seeds (T2 generation) in hygromycin solution to see the segregation ratio of 7 events. Did anyone tried this before?? Will appreciate any comments on...

18 September 2018 4,250 2 View

How to purify recombinant protein from pellet?

Hi all. I want to purify a his-tagged recombinant protein. But when I try to check the recombinant-protein by sds page/western blotting, I see it in bacterial cell debris (the first pellet after...

24 February 2017 1,601 16 View

Random mating, population genetics?

Question : In a large random mating population, there are initially 91 percent dominants for a monogenic trait. a. If there would be 20% selection against the dominants, what would be the...

09 February 2017 7,184 3 View

How to get the cDNA sequence from genomic sequence for different genotypes?

Hi all I have genomic sequence of different rice genotypes. I want to compare it at protein level. to serve the purpose I need to convert genomic to cDNA sequence. Can anyone suggest me any online...

18 October 2016 7,278 3 View

Which is the preferred pathway of Xanthine formation in plants?

Which is the major/preferred substrate for Xanthine formation in plants? Guanine, Adenine or Hypoxanthine? Will appreciate your comments with scientific proof, but this is also open for...

01 January 1970 535 0 View

Does CA repeat sequence in 5'UTR inhibit/control translation?

Hi, I am working on a gene where I recently found Insertion of CACA in 5'UTR of one of the Rice genes I am working on, with strong phenotypic differences. Here's the sequence -...

01 January 1970 9,045 0 View