Derya Kabacaoglu

3 Questions 8 Answers 0 Followers

Questions related from Derya Kabacaoglu

Why do the western samples which gave equal loading control at first time do not give equal loading control bands when I reuse them?

Hi, I have a question regarding reuse of western blot cell lysates. When I do the b actin control of the lysates at first right after bradford assay, the loading control of all lysates seem equal....

02 February 2016 8,625 8 View

Why do I have an unexpected band at 400bp in my negative control PCR? Can primer dimers be the reason for that?

I am performing a PCR reaction with phusion which gives an expected band around 400 bp. I am also getting the same band around 400bp in my negative control which has no template in it with a pale...

10 October 2014 6,698 26 View

Why does my flag-tagged exogenous protein give multiple bands?

In my western blot analysis of both total cell lysate and immunoprecipitated samples, I saw 2 bands. The expected band is around 15kDa but I see another additional band around 25kDa in every...

02 February 2014 409 3 View