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I've tried IFN-gamma at 200 IU/ml but not seen any upregulation in surface MHC class I in Vero cells. This concentration typically gives me a 3-fold increase in surface expression in porcine...
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I'm trying to evaluate the effect of virus infection on MHC class I expression, and am using GAPDH as my housekeeping gene, which should be showing standard levels of expression across my samples....
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