Deo Singh

4 Questions 8 Answers 0 Followers

Questions related from Deo Singh

How to target parasympathetic neurons in the pancreas of the Rat?

I am studying the effect of parasympathetic stimulation on the islet beta cells. Since, the parasympathetic innervates the pancreas and it has been hypothesized that stimulation of parasympathetic...

01 January 2018 9,095 0 View

What is the physiological concentration of E-cadherin?

I wanted to to know when E-cadherin is expressed in normal cells what is its concentration.

10 October 2016 9,196 3 View

Why the E-cadherin cis dimers were never observed using direct biophysical methods while these were observed for EC1 domain crystal structure?

The crystal structure of EC1 domain of N-cadherin was determined by Shapiro et al. in 1995. In that structure he observed cis dimers. Although many biochemical methods have provided evidence of...

10 October 2016 6,538 1 View

Do I need to add Sampling buffer and heat it at 70C for 10 minutes before running the blot for a cross-linked lysate?

Hi, I have cross-linked the E-Cadherins using DTSSP. I am planning to run the western blot experiment to see if it forms higher order oligomers. I am not sure if I should use Sampling buffer and...

12 December 2015 6,741 7 View