Denise Kemler

3 Questions 24 Answers 0 Followers

Questions related from Denise Kemler

Recommendation for molarity of Citrate buffer to use with C2C12 myotubes?

I am planning an experiment using TTX on C2C12 myotubes. The TTX should be reconstituted in citrate buffer pH4.8. I am now trying to find out which molarity of citrate buffer can be used for C2C12...

07 July 2018 7,446 1 View

How to immobilize isolated fibres ?

Hey all I want to image and analyze the movement of satellite cells on isolated fibers. My Setup is fixed everything works unless that I'm not able to immobilize my fibre in a way that it's not...

09 September 2016 4,370 3 View

How can I prevent my C2C12 cells from detaching from a collagen coated coverslips?

Hello everyone! I have a problem with my C2C12 cells. For imaging purposes I have to differentiate C2C12 cells on coverslips. I coated these either with Laminin (2% in PBS) or Collagen I (10µg/ml...

10 October 2014 9,712 7 View