Dengya Zhang

5 Questions 5 Answers 0 Followers

Questions related from Dengya Zhang

How many colonies should I get to make sure that each variant in the library is included?

I am going to construct a library in E. coli, and the number of variants is about 107. To make sure that every variant in the library is included, each plasmid should be transformed into E. coli...

23 May 2020 7,872 1 View

How frequently will homologous recombination (HR) happen in E. coli with λ-RED system and a DSB?

I am going to construct a library where several genes of a pathway are contained and each gene is expressed in different level. The number of variants in this library is about 107. I want to...

23 May 2020 7,624 3 View

How efficient can a 12k plasmid be transformed into E. coli BL21star(DE3)? Will it be as high as 10^7 cfu/μg?

I am going to construct a library with plasmid whose size is around 12k, and the number of variants is about 107. Will the transformation efficiency be high enough for the construction? Will...

23 May 2020 1,655 0 View

What may cause a gene not to be expressed in chromosome?

I have successfully intergated several genes into the chromosome of E.coli to produce an oligosccharide. Theoretically, this oligosaccharide can be produced in a scale of at least 0.3g/L, and this...

21 July 2019 6,482 2 View

What may cause a gene not to be expressed in chromosome?

I have successfully intergated several genes into the chromosome of E.coli to produce an oligosccharide. Theoretically, this oligosaccharide can be produced in a scale of at least 0.3g/L, and this...

07 July 2019 4,859 2 View