Deepak Iyer

6 Questions 12 Answers 0 Followers

Questions related from Deepak Iyer

I have 2 questions related to piRNA analysis: (A) Selection between piRNAs with highly similar sequences. (B) Mapping of potential piRNA targets?

So I am performing a biomarker (piRNA based) study in a human solid cancer. Based on the initial analysis, I have shortlisted a few piRNAs - but I am stuck with two issues: 1. A few piRNAs have...

03 March 2019 4,348 1 View

How can I access tumor adjacent normal tissue data (for colorectal cancer) from websources such as TCGA or Geo for microenvironment based miRNA study?

My research specifically looks at the colorectal tumor's adjacent normal tissue and its small RNA (mainly miRNA) expression and its role as a prognostic marker. Currently I wish to interrogate...

01 January 2018 566 2 View

How long do lentiviruses remain active (do not disintegrate) in the culture media?

Lentiviral Transduction related: I wanted to understand the average time period that you can expect Lentiviruses carrying your target gene remain active within the culture media of the target...

09 September 2017 9,883 1 View

Evaluating user responses for miRNA profiling by qPCR using the polyadenylation strategy?

I am looking to profile miRNAs (via qpcr) within clinical samples (tissue & blood) and have been fascinated towards using the polyadenylation based strategy using SYBR green primarily because...

06 June 2015 6,821 5 View

What is the optimum read length required for a illumina based small RNA sequencing?

I am planning to pursue a small RNA sequencing project, however, I am a bit under confident in choosing the correct read length for a illumina hiseq based small RNA sequencing. The options that I...

07 July 2014 4,025 6 View

What is the importance of laser microdissection in tissue profiling prior to RNAseq?

I am trying to build an miRNA profile from a number of cancer tissue/normal samples via RNAseq. What I needed to know is, how much is it essential to sort the tumor or normal population of the...

05 May 2014 1,549 5 View