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Questions related from Deepak Iyer
So I am performing a biomarker (piRNA based) study in a human solid cancer. Based on the initial analysis, I have shortlisted a few piRNAs - but I am stuck with two issues: 1. A few piRNAs have...
03 March 2019 4,346 1 View
My research specifically looks at the colorectal tumor's adjacent normal tissue and its small RNA (mainly miRNA) expression and its role as a prognostic marker. Currently I wish to interrogate...
01 January 2018 562 2 View
Lentiviral Transduction related: I wanted to understand the average time period that you can expect Lentiviruses carrying your target gene remain active within the culture media of the target...
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I am looking to profile miRNAs (via qpcr) within clinical samples (tissue & blood) and have been fascinated towards using the polyadenylation based strategy using SYBR green primarily because...
06 June 2015 6,817 5 View
I am planning to pursue a small RNA sequencing project, however, I am a bit under confident in choosing the correct read length for a illumina hiseq based small RNA sequencing. The options that I...
07 July 2014 4,021 6 View
I am trying to build an miRNA profile from a number of cancer tissue/normal samples via RNAseq. What I needed to know is, how much is it essential to sort the tumor or normal population of the...
05 May 2014 1,549 5 View