Protein transfer issues from SDS-PAGE gel to NC

My protein are of 30-60 kD range. I use 9% gels and transfer them on Amersham's Semi dry unit (0.8mA/cm.sq for 1hr). I use 14.4g Glycine, 3.03 g Tris base and 0.1% SDS per litre of MQ water. My...

03 March 2013 2,785 6 View