Deepa Nair

2 Questions 4 Answers 0 Followers

Questions related from Deepa Nair

How to store the coated ELISA plates to increase shelf life upto 6 months?

Hi I am standardising and in house ELISA (indirect ELISA). I have coated my ELISA plate (NUNC maxisorp) with virus diluted in carbonate-bicarbonate buffer (pH- 9.6)-- overnight at 4C. Blocked...

03 March 2019 1,755 2 View

Problems associated with coating Hepatitis B vaccine as an antigen?

I am trying to develop an ELISA to measure antibodies to Hepatits B surface antigen in human serum.My detection antibody is an anti-human IgG-HRP. I getting a proper sigmoidal standard...

07 July 2016 8,753 3 View