Dee Aa

4 Questions 4 Answers 0 Followers

Questions related from Dee Aa

What techniques do you use for hESC colony picking?

I'm growing hESC cells that are recovering from a transfection in a 6 well plate. They form colonies but I find it quite difficult to pick the colonies. When I detach them using a colony picking...

04 April 2018 9,419 2 View

Why do my hESCs die after I've added 5% Pen/Strep?

The cells were growing slower than normal but they were still growing and forming colonies after transfection. However after I added 5% Pen/Strep the cells around the edges start to detach and...

04 April 2018 9,581 2 View

Why are my hESCs not growing in 48 or 96 well plates after colony picking post-transfection?

Hi, I'm currently transfecting my cells using 4D Amaxa electroporation and the cells recover beautifully post-transfection. However no matter how many times I try or whatever method I use the...

04 April 2018 9,744 4 View

Why does my hESC die after plating into 24 well plates?

I use 150k cells per well and these cells were plated after transfection. It's not due to the transfection as cells recover just fine when in 6-well plates. But everytime I work with smaller...

04 April 2018 1,509 3 View