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Questions related from Debopriya Roy
Hello everyone I want to study GEFx and its binding with GTPase. Rxn as such: GEFx = 2uM GTPase = 1uM buffer = 10mM tris, 150mM nacl, 5% gly, 2mM bME (buffer same for both above proteins) + 10mM...
05 May 2018 2,184 1 View
Hello everyone I want to study GEFx and its binding with GTPase. Rxn as such: GEFx = 2uM GTPase = 1uM buffer = 10mM tris, 150mM nacl, 5% gly, 2mM bME (buffer same for both above proteins) +...
05 May 2018 9,010 0 View
hello everyone I am trying to express a protein with SUMO tag which used to express initially in buckets by a labmate in BL21 RIL at 500mM iptg induction overnight at 18*C. but when i tried...
01 January 2018 5,134 5 View
Greetings everyone I am trying to mix lysates of two interacting proteins. 1. P1-TEV-SUPERFOLDING GFP-HIS (Using superfolded gfp to improve solubility) size should come 70KD 2. P2-uncleavable HIS...
05 May 2017 2,669 6 View
Hi everyone While working with a protein in P3E construct, the protein seems soluble in presence of CHAPs in lysis buffer (also screened Tween 20, triton 100, DDM) detergent, i purified it using...
01 January 2017 6,689 7 View
hello friends I have recently started with Insect cell culture work. I transfected SF9 cells in 6 well plate with Bacmid containing my GOI. Even though in literature its metnioned to use P2 virus...
12 December 2016 1,352 2 View
hello friends recently we have got StrataClone PCR cloning kit to clone a gene of interest about 6.1 kb size. I used LA Taq DNA polymerase for PCR, Gel purified the bands, incubated it with Taq...
08 August 2016 9,322 5 View
I have tried cloning my GOI in topo TA cloning vector with given protocol provided with the kit. But everytime i get colonies in Kana plates which tend to be false +ve. The Topo kit worked...
07 July 2016 4,752 3 View
I have been trying to clone a 6.2Kb gene in CMV vector, but i am stuck at the initial stage itself. for PCR i have used LA Taq DNA polymerase/ Accu Taq LA DNA polymerase which produce intense...
05 May 2016 4,680 2 View
