Deanna M Santer

5 Questions 8 Answers 0 Followers

Questions related from Deanna M Santer

Is there a way to calculate ddCts/fold changes if your gene of interest is undetectable in unstimulated but then detectable after stimulation?

Hi all This must have happened to others before. For example an IFN stimulated gene like MX1 goes from undetectable (>40 cycles) but then after IFN alpha stimulation the Ct is now 28. Both...

27 March 2018 9,469 4 View

What is the best way to broadly screen for signaling pathways being turned on by a certain stimulus?

We would like to be able to screen multiple signaling pathways at once with primary cells after stimulating with whatever stimulus we choose. Doing tons of western blots is not possible so hoping...

18 July 2017 4,934 4 View

What brand tissue culture plate is best for human monocyte culturing and what density do you plate them?

When you purify human monocytes from PBMCs (magnetic selection) we have noticed some differences in the brand of tissue culture plates used. Has anyone compared different brands like BD to Corning...

08 February 2017 3,076 4 View

What is the best way to measure virus specific IgG from in vitro human cell stimulations?

We are setting up a variety of virus stimulations as positive controls for adding to human PBMCs. My goal is to measure IgG induced by the virus so while total IgG is easy to measure via ELISA,...

24 June 2014 2,570 4 View

Has anyone been successful using filter plates to surface stain/wash human PBMCs for flow cytometry?

I am curious if anyone has been successful using a vacuum manifold to wash FACS samples instead of centrifugation to save lots of time if stain samples in a 96 well millipore filter plate? We...

25 March 2014 9,436 5 View