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Questions related from Dayana Cheong
My current protein concentration is 13mg/ml. To load 30ug protein for SDS PAGE, only 2.15uL is needed, can i load 2.15uL sample + 2.15uL 2X Laemmli Buffer = 4.3uL in the well or do I dilute the...
29 January 2024 1,978 2 View
Is the MTT result for 72 hour treatment valid if I treat the cells with the drug for 15 days? The cells are exposed to the drugs for far more than 72 hours so how does 72hr MTT treatment result...
25 September 2023 1,609 3 View
Can i use water to dilute my plant extract and treat 3T3L1 cells or i should dilute the extract using the culture media? Right now the dilution is 1:1 part of extract in water : media. Will this...
23 August 2023 4,436 2 View
Does the SPD for SPD-20A UV/VIS detector in HPLC means Silicon PhotoDiode?
18 August 2023 6,287 0 View
Im going to perform MTT assay on 3T3-L1 preadipocytes. After seeding cells in 96 well plate, do i have to wait 48 hours post confluent, then replace with treatment then only MTT solution? Or just...
17 August 2023 8,176 5 View
Im currently differentiating 3T3-L1 cells in 96 well. After changing to differentiation media for 72 hours, I replace with insulin media, but the lipids droplets that formed disappears. until...
19 June 2023 4,497 2 View
I am currently culturing the 3T3-L1 cells, and trying to differentiate them. What happens if I change to differentiation media immediately after confluence? Why do we have to wait 2 days...
05 June 2023 7,996 1 View
Can i use water to dilute my plant extract and treat 3T3-L1 cells or i should dilute the extract using the culture media? Right now the dilution is 1:1 part of extract in water : media. Will this...
01 January 1970 3,802 2 View
