Dayan Lozano

6 Questions 4 Answers 0 Followers

Questions related from Dayan Lozano

Is it possible to do a PCR using primers with Tm ° C=78.9?

I will do a PCR for cloning, for this reason the primers have the sequence of the ERs at their ends, for this reason the Tm are high. It is possible to do a PCR with these high Tm primers and what...

04 April 2019 1,292 5 View

What's the best human macrophage cell line for estimation of the diferential gene expression of S. aureus during a infection celular model?

I need to compare the gene expression of S. aureus during infection celular model.

07 July 2018 1,941 2 View

I need a dye for the quantification by flow cytometry-based assay or cholorimetric method-based, can somebodyhelp me please?

I need to make the growth bacterial curve

10 October 2017 8,445 5 View

Hello. Anybody have a DNA from sample Entamoeba polecki?

I need to standarize a PCR method for to identify the 18s rRNA unid from this. Thanks!

08 August 2017 3,803 1 View

Do you know any protocol for the isolation of phages for staphylococcus aureus from nasal swap?

I tried to isolate phages from nasal swab using the Kropinsky´s 2009 protocol with LB + CaCl2 and using the strain RN4220 host. You know others protocols for this purpose.

06 June 2017 6,429 1 View

When phage DNA phages are purified, how do we know if this is the phage DNA and not the host bacteria?

When we try to purify phage DNA, we used a host bacteria, how do we differentiate that corresponds to the phage DNA itself and not the bacteria? Which molecular method could be used because i do...

01 January 2016 7,433 3 View