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Questions related from Dayan Knox
So I have been doing IHC on sections mounted on glass slides for a while. Lots of advantages to this, but one disadvantage is double labeling of proteins in single neurons. For this part of my...
10 December 2019 6,548 2 View
So as luck would have it I may be in the market for purchasing a fluorescent microscope. I really life the EVOS fl systems, but was wondering if anyone had experience (good or bad with it). I...
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So I typically use western blot and IHC (near infrared imaging in both) to assay proteins in brain tissue. Sometimes I used DAB reactions as well. However, I've had the need to switch to Alexa...
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So life claims this new system will greatly improve westerns. Here is a link to the system (below). Is this true? Anyone has experience with this.
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So I am considering using the trans-blot turbo system over the traditional tank system. Transfer of proteins using the tank system can be very efficient, but takes long and bubbles are always a...
29 May 2013 4,463 29 View
For those of you who don't know about it, you can find information here:...
14 May 2013 628 3 View
So I was wondering what is the best method for transferring proteins from gels onto nitrocellulose or pvdf membranes. Semi dry or standard chamber? I have only ever used standard chambers, but was...
19 December 2012 1,865 33 View