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Questions related from Dayan Knox
So I have been doing IHC on sections mounted on glass slides for a while. Lots of advantages to this, but one disadvantage is double labeling of proteins in single neurons. For this part of my...
10 December 2019 6,547 2 View
So as luck would have it I may be in the market for purchasing a fluorescent microscope. I really life the EVOS fl systems, but was wondering if anyone had experience (good or bad with it). I...
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So I typically use western blot and IHC (near infrared imaging in both) to assay proteins in brain tissue. Sometimes I used DAB reactions as well. However, I've had the need to switch to Alexa...
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So life claims this new system will greatly improve westerns. Here is a link to the system (below). Is this true? Anyone has experience with this.
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So I am considering using the trans-blot turbo system over the traditional tank system. Transfer of proteins using the tank system can be very efficient, but takes long and bubbles are always a...
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For those of you who don't know about it, you can find information here:...
14 May 2013 627 3 View
So I was wondering what is the best method for transferring proteins from gels onto nitrocellulose or pvdf membranes. Semi dry or standard chamber? I have only ever used standard chambers, but was...
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