Dayan Knox

7 Questions 29 Answers 0 Followers

Questions related from Dayan Knox

Equipment for immunohistochemistry in free floating sections?

So I have been doing IHC on sections mounted on glass slides for a while. Lots of advantages to this, but one disadvantage is double labeling of proteins in single neurons. For this part of my...

10 December 2019 6,618 2 View

What is the best cost effective confocal microscope?

So as luck would have it I may be in the market for purchasing a fluorescent microscope. I really life the EVOS fl systems, but was wondering if anyone had experience (good or bad with it). I...

27 September 2017 8,944 6 View

What's a good protocol for using Alexa Flour antibodies for IHC in brain tissue?

So I typically use western blot and IHC (near infrared imaging in both) to assay proteins in brain tissue. Sometimes I used DAB reactions as well. However, I've had the need to switch to Alexa...

10 August 2016 1,843 5 View

Does anyone have any experience using the iBind Western system?

So life claims this new system will greatly improve westerns. Here is a link to the system (below). Is this true? Anyone has experience with this.

21 January 2015 5,813 6 View

Does anyone have experience using the Trans-Blot® Turbo™ Transfer System from Bio-rad?

So I am considering using the trans-blot turbo system over the traditional tank system. Transfer of proteins using the tank system can be very efficient, but takes long and bubbles are always a...

29 May 2013 4,526 29 View

Just curious what everyone thought about the initiative from the NIMH referred to as Research Domain Criteria (RD0C)?

For those of you who don't know about it, you can find information here:...

14 May 2013 669 3 View

For Western Blots, should I transfer proteins using standard chamber or switch to a semi-dry transfer method?

So I was wondering what is the best method for transferring proteins from gels onto nitrocellulose or pvdf membranes. Semi dry or standard chamber? I have only ever used standard chambers, but was...

19 December 2012 1,909 33 View