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Questions related from Daxing Gao
I have been trying to measure mouse plasma/serum IL-12 by multiplex TH1/TH2 cytokine kit (MSD). And the IL12 concentration from healthy mouse is around 1ng/mL from my measurement. For other...
08 August 2014 9,404 4 View
I am trying to stain mouse spleen cells for Tbet. I wonder what percentages of CD4+ T cells or CD8+ T cells are Tbet positive in spleen of wild type B6 mice? Thanks in advance!
07 July 2014 7,961 3 View
I am trying to do IFN-γ intracellular staining of mouse spleen cells and PBMC. I will stimulate cells with PMA and ionomycin first. I want to know what percentages of CD4+ T cells or CD8+ T cells...
07 July 2014 9,354 6 View
Recently I have been working with detecting serum IL12 by ELISA. Based on that result, the sera from healthy mice have around 10000~20000pg/mL IL12p40/p70. But when I searched the literature,...
06 June 2014 6,927 2 View
When I stained mouse PBMCs, I saw there was a population of Ly6c+CD11b- cells (>10%) and these cells express CD86. From the FSC and SSC, I can tell they are not lymphocytes. Does anybody have an...
02 February 2014 8,709 7 View
