Dawid Zyla

5 Questions 15 Answers 0 Followers

Questions related from Dawid Zyla

What are the best 3 different reporter proteins as fusion partners for structural studies?

Hello, I am looking for 3 different reporter proteins (fluorescence / chemiluminesence / enzymes) which could have a fused tag on C-terminus (N -reporter protein - tag - C). I want to use them for...

08 July 2016 5,170 2 View

Why protein refolding rate depends on observed wavelength?

Hello, I am working with a small, single subunit protein with relatively slow refolding rate (~20 min). I have noticed that after thermal unfolding protein refolds without any precipitation. I...

14 March 2016 6,904 7 View

What could be the unidentified small molecule density in protein electrom map?

Dear all, I have got a protein crystals which diffracted very well (~0.9A) and during solving the structure I have noticed that around my protein in few places I have a electron density of the...

13 October 2015 7,073 12 View

Can someone provide me with some advice on using BS3 crosslinker and DTT buffer?

I would like to perform a crosslinking experiment using BS3 crosslinker. My protein has 3 cysteines, but no S-S bridge, so I have to keep my protein in buffer with 5mM DTT. Can I perform the...

17 July 2013 9,844 9 View

Help with BS3 crosslinking between two proteins

I would like to check interaction between two proteins. Previously it was reported that 2 proteins I work on interact. I have read a publication where was used BS3 crosslinker. They mapped all Lys...

03 May 2013 9,068 2 View