David Carmona-Berrío

2 Questions 3 Answers 0 Followers

Questions related from David Carmona-Berrío

Why does my primer prefers to anneal where it's not 100% complementary?

Hi researchers, the thing is that I've been trying to amplify a PCR product of 700 bp long and when I run the electrophoresis gel to verify the amplification result all I get is a PCR product of...

03 March 2020 3,873 5 View

Someone have used pET101/D-TOPO from Invitrogen? I have problems trying to ligate my PCR insert, any advice?

Hello researchers, Here's the thing. I've this expression kit (Champion™ pET101 Directional TOPO™ Expression Kit REF 45-0175) and I've read about the protocol provided by Invitrogen. Where it...

10 October 2019 1,021 3 View