David Sassoon

5 Questions 33 Answers 0 Followers

Questions related from David Sassoon

What is the best system (and company if possible) for viral Cre delivery to primary (proliferative) cells in culture?

We have the conditional allele mouse that has been tested with several Cre and CreER mice and it works. However, we can move faster if we just infect Cre directly into the cells but we need high...

19 May 2015 241 3 View

Is there evidence for non-satellite cell resident muscle progenitors?

On ResearchGate, there has been a very collegial discussion about the role of satellite cells during muscle hypertrophy. A number of us also cited increasing evidence that non-satellite cells play...

21 May 2014 8,985 29 View

Are both alleles always active in 'normal' gene transcription?

We are performing FISH directly on cells to determine the number of alleles involved in active transcription. We see that even for 'non-imprinted' genes, only about 20% of cells that contain...

03 September 2013 6,802 31 View

Centrally located nuclei after regeneration?

How long does it take for the skeletal myofiber nuclei to regain a peripheral location after regeneration? More specifically, muscle appears completely regenerated after 2 weeks following...

26 July 2013 6,872 3 View

If siRNA and/or shRNA knock down gene expression are at undetectable levels, are these results less significant than using a conditional KO mouse?

This question was asked to me by an editor of a journal. I think both are valid models but the editor feels that KO mice are better.

12 December 2012 8,568 17 View