David Dayanidhi

6 Questions 6 Answers 0 Followers

Questions related from David Dayanidhi

Is it possible to conjugate an NHS ester to the N-terminus of a peptide ON RESIN?

So i have a few peptides (synthesized by SPPS and not cleaved from resin) and I want to add a maleimide label. We have NHS-PEG-Maleimide and from literature it is evident that the free amine group...

19 July 2024 3,949 3 View

How reliable is ninhydrin test in solid-phase peptide synthesis?

Hey everyone! I'm doing peptide synthesis on Rink amide resin and sometimes I need to add amino acids with secondary amines or beta amino acids. Normally I synthesize 35 to 40 mer peptides. As a...

22 May 2024 8,974 0 View

Is it possible to narrow down peptide fragments using MWCO columns?

Hey everyone! I have a few peptide sequences synthesized using SPPS and all of them are in the M.wt range of 6 to 8kDa. I've done MALDI MS and found out that I do have the desired peptide peak but...

14 May 2024 5,794 2 View

Need clarifications on the preparation, purification and concentration measurement of calf thymus DNA solution?

My objective is to prepare pure CT-DNA solution. For that, I have taken a known amount of CT-DNA fibers (sodium salt) in a 10 mM Tris buffer solution and stirred at low rpm at 4 degrees overnight....

23 November 2022 8,955 1 View

Is there a way to convert an Ir(III)-PF6 coordination complex to a water-soluble Ir(III)-Cl coordination complex without using sephadex?

I have synthesized a hexafluorophosphate salt of and Ir(III) cyclometalated complex. I have tried using a DEAE anion exchanger sephadex but that did not convert the PF6 to Cl. Is there another way...

23 September 2020 5,706 0 View

Is there any way to evaporate a high boiling point solvent to retrieve a dissolved product?

I am using solvents like 2-ethoxyethanol, DMF or toluene at times for synthesis and since they are all high boiling point solvents, it is very very difficult to evaporate them in a rotary evaporator.

23 March 2020 2,374 3 View