David Carmona-Berrío

3 Questions 3 Answers 0 Followers

Questions related from David Carmona-Berrío

Smear bands during colony PCR to identify mi gene of interest? but one positive?

Hey researchers! So I was using a pET 101/D-TOPO (From Invitrogen) vector of old storage (5 years on -20 ºC) so the enzymatic efficiency of the TOPO has been reduced obviously, hoping it to work...

29 October 2020 3,410 4 View

I'm having trouble trying to transform my E. coli BL21 (DE3) what am I failing at?

Hi researchers! I made my own E. coli BL21 (DE3) competent cells with CCMB80 buffer working with an 0,52 OD. And the day after I performed my transformation protocol in this way: 1- 20 ng of...

10 September 2020 2,035 4 View

I was getting a PCR product of 1000 pb instead 700 bp! and now I'm getting the 700 bp normally. How that can be possible?

Hi Researchers, Let me tell you that I was trying to isolate a gene of interest (700 bp) from a Cosmid as a template (36 kbp) and I got a PCR product of 1000 bp instead of my gene of interest!...

14 July 2020 8,725 8 View