David Bernal-Estevez

2 Questions 3 Answers 0 Followers

Questions related from David Bernal-Estevez

Hi, Can IFNg have a pro-tumor effect in breast cancer or other tumors?

I`m analyzing some DCs that produce high amounts of IL-12 that can induce strong T cell response (IFNg production), but in some tumors this can be a non desired result. my model is in breast...

26 October 2017 1,279 2 View

Does anybody know how can I improve monocyte purification by plastic adherence from total human PBMCs, anyone has a detailed protocol?

Hi, I use buffy coats from blood units of healthy donors, I culture 1.2x10^8 PBMCs in 10mL of AIM-V after 2h adherence in a T75 flask (Polystyrene - tissue culture treated) the yields of monocytes...

26 February 2015 3,170 4 View