Does anybody know how can I improve monocyte purification by plastic adherence from total human PBMCs, anyone has a detailed protocol?

Hi, I use buffy coats from blood units of healthy donors, I culture 1.2x10^8 PBMCs in 10mL of AIM-V after 2h adherence in a T75 flask (Polystyrene - tissue culture treated) the yields of monocytes...

26 February 2015 3,151 4 View