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I kept 10 mL vial in ice bucket in cold room (2-8) maintaining 4 to 5 deg C. it thawed properly. Aliquotes (1mL) of that vial i kept back in -20. When i need one aliquot and i thawed in similar...
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I am using SH-SY5Y cells for hypoxia in a hypoxia chamber which I got from stem cell technology. I am using commercial pre mixed cylinder (2% O2, 5% CO2 and balance N2). I am using hanks balanced...
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