Dario Saczko-Brack

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Questions related from Dario Saczko-Brack

Lipid structures disappear upon negative stainging. Does anyone knows this effect?

Dear all, I have large, long lipid structures (several µm up to mm) that are present on a carbon coated 400mesh copper grid. My aim is to image these in the TEM. Using confocal microscopy I can...

14 December 2020 1,681 3 View

Cryo-EM Vitrobot settings for large lipid structures. How can I reduce ice thicknes?

Dear all, I have an issue figuring out good plunging conditions for large lipid structures: I have carbon coated C-Flat 2/2 400mesh copper grids with large (elongated) lipid structures on it....

14 December 2020 6,854 2 View