Dario Melgari

8 Questions 2 Answers 0 Followers

Questions related from Dario Melgari

How to reduce leakage current in mouse DRGs patch-clamp?

I'm patching some neurons isolated from mouse DRGs to record low and high voltage-activated calcium currents. All the patched cells have a leakage current of at least -400 pA. I'm using the...

11 February 2025 2,563 0 View

Does anyone have a cell line stable expressing CaV3.2 channel for electrophysiological studies that can share?

Hello. I'm looking for a cell line stable expressing CaV3.2 (CACNA1H, T-Type) ionic channels. It will be for eletrcophysiological studies (manual and automated patch-clamp). Thank you in advance!

01 December 2021 4,919 2 View

H9c2 culture/seeding protocol for whole-cell Patch-Clamp?

Does anyone have a detailed and reliable protocol for culturing, seeding and preparing H9c2 cells for whole-cell patch-clamp? What's the best practice to prepare H9c2 for single cell...

26 January 2021 2,470 0 View

Why don't I see any current in cell-attached patch-clamp?

I am trying to record some Nav1.5 current from adult rat ventricular cardiomyocytes in the cell attached configuration. I am working at 37C with extracellular (bath) isotonic potassium in order to...

12 June 2020 2,382 0 View

How can I hide/remove unwanted Analog OUT channels in Clampex?

Hello everyone, I want to remove the Clampex Analog OUT channels that I am not using but I cannot find any way to do so. As you can see from the images, preview of the waveform shows all the...

05 November 2019 3,791 5 View

Is there a clear protocol for lentivirus infection of cardiomyocytes?

Hello everyone, I am looking for a clear protocol for the lentiviral infection of rat cardiomyocytes (atrial and ventricular). The general literature on the topic indicates the usage of...

05 June 2019 6,161 0 View

How can I analyse fluorescence images of double stained cells?

I have a set of cells double stained in this manner: first the cells has been live-stained to visualize the protein of interest at the membrane (named: SURFACE). Then, cells have been fixed and...

24 July 2018 9,193 6 View

Is it possible to purify clathrin-coated vesicles from atrial cardiomyocytes? If so, can anyone suggest a good protocol?

I'm thinking about to possibility of purify CCVs from atrial CMs (rat). The available protocols in the literature are focused on cell lines or on tissues from organs such as brain and liver. Does...

26 January 2018 8,995 0 View