Dario Melgari

4 Questions 2 Answers 0 Followers

Questions related from Dario Melgari

Does anyone have a cell line stable expressing CaV3.2 channel for electrophysiological studies that can share?

Hello. I'm looking for a cell line stable expressing CaV3.2 (CACNA1H, T-Type) ionic channels. It will be for eletrcophysiological studies (manual and automated patch-clamp). Thank you in advance!

01 December 2021 4,890 2 View

H9c2 culture/seeding protocol for whole-cell Patch-Clamp?

Does anyone have a detailed and reliable protocol for culturing, seeding and preparing H9c2 cells for whole-cell patch-clamp? What's the best practice to prepare H9c2 for single cell...

26 January 2021 2,431 0 View

Is there a clear protocol for lentivirus infection of cardiomyocytes?

Hello everyone, I am looking for a clear protocol for the lentiviral infection of rat cardiomyocytes (atrial and ventricular). The general literature on the topic indicates the usage of...

05 June 2019 6,131 0 View

How can I analyse fluorescence images of double stained cells?

I have a set of cells double stained in this manner: first the cells has been live-stained to visualize the protein of interest at the membrane (named: SURFACE). Then, cells have been fixed and...

24 July 2018 9,136 6 View