Danuta Sastre

2 Questions 7 Answers 0 Followers

Questions related from Danuta Sastre

Plasmid co-transfection: if one plasmid gets in, will the other ones too?

I am co-transfecting 3 plasmids, respecting the maximum amount of DNA per volume and using a established DNA:cationic reagent ratio in 293T cells. One of my plasmids has a fluorescent marker and...

11 November 2017 9,655 3 View

What could cause transformed E. coli to form colonies in agar, but fail to grow in liquid culture?

I'm using NEB HiFi DNA Assembly, an equivalent to Gibson Assembly from New England Biolabs, to clone 2 fragments (1491bp and 3756bp) into a 8.8kb backbone (lentiviral/mammalian expression)....

09 September 2017 4,556 14 View