2 Questions 7 Answers 0 Followers
Questions related from Danuta Sastre
I am co-transfecting 3 plasmids, respecting the maximum amount of DNA per volume and using a established DNA:cationic reagent ratio in 293T cells. One of my plasmids has a fluorescent marker and...
11 November 2017 9,662 3 View
I'm using NEB HiFi DNA Assembly, an equivalent to Gibson Assembly from New England Biolabs, to clone 2 fragments (1491bp and 3756bp) into a 8.8kb backbone (lentiviral/mammalian expression)....
09 September 2017 4,564 14 View