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I made a D64V mutation on integrase for creating a integrase deficient lentivirus.Three day post-transduction, I noticed that not many cells were transduced based on the number of fluorescent...
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Hello. We have integrated the EGFP gene into immortalized human fibroblast using lentivirus. The gene is flanked by loxp. And now we are trying to knock this gene out using Cre plasmid by...
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