Doxycycline concentration for Tet-on induction in primary culture of neurons?

Hello, Does anyone used TetR-based Tet-on expression system in E18 rat hippocampal neurons? I want to transfect the hippocampal neurons at 0 d.i.v and treat them with doxycycline at 1 d.i.v. Does...

13 December 2021 9,467 1 View