Daniela Ugalde-Araya

4 Questions 7 Answers 0 Followers

Questions related from Daniela Ugalde-Araya

Please could anyone guide me on how to edit (width/color/lines etc) a pedigree using kinship2 in R?

I am working with an extended family, I already have the pedigree but I want to edit the figure because it is huge, so the lines are very thin and the squares and circles are very small. I thought...

02 February 2019 4,933 3 View

Can anyone explain me wich is the best protocol to evaluate % of positive cells in a immunofluorescence photo (whichever marker)?

I have seen many articles with bar graphs on different percentages of positive cells from immunofluorescent photos, but I need to know if to create these graphs it is only necessary to observe the...

01 January 2018 8,086 4 View

To quantify fluorescence in image J (Fiji) is it necessary to have the photos in TIFF format?

My pictures are in jpg format, I need to know if it is possible to quantify fluorescence of S100B and GFAP in cells. And wich is the best way to do this, using mean gray value or integrated...

11 November 2017 9,742 5 View

What is the best protocol to detect ROS in cell culture using CM-H2DCFDA?

Recently I have been working on ROS production studies using the dye CM-H2DCFDA. On live cells (astrocytes) the concentration of dye is 2.5uM and incubation for 30min. The problem is that the dye...

09 September 2017 8,682 11 View