Daniela De Martino

4 Questions 15 Answers 0 Followers

Questions related from Daniela De Martino

Has anyone done a double IF with HA antibody and flag-antibody? Which couple of antibodies can you suggest to me?

I'm trying to find a great couple of antibodies to use in an immunofluorescence experiment to study the localization of proteins....thanks to anybody would help...

08 August 2014 8,857 2 View

Can anyone suggest a good method of protein purification to conduct studies of crystallography?

I need to use a fast method to obtain a protein pure enough for preparation in crystallografic studies.

10 October 2013 3,830 11 View

Does anyone have any suggestions on how to prevent proteolysis during these three steps of purification (a His-tagged, ion exchange, gel filtration)?

I'm trying to obtain a good preparation of my protein for crystallization analysis, but after the first step of purification (his-tag) I always have protein stability problem.

10 October 2013 6,593 12 View

How pure can a purified recombinant protein with Ni-column method and ionic exchange chromatography be?

Let me explain my problem, after a good yield of ionic exchange chromatografy (used as a second step of purification) my protein that should bind to another protein expressed at the same time,...

10 October 2013 833 15 View