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Questions related from Daniela Eletto
Does anyone know whether after knockingout a gene by Crispr, cells can be recomplemented with the same exogenous protein?
07 June 2015 7,406 4 View
I am trying to grow an unstable plasmid (PERK, transmembrane protein of the endoplasmic reticulum) but the amount of DNA that I usually get from bacterial cultures is always too low (sometime...
13 September 2013 6,416 10 View
We would like to find an alternative to the Lipofectamine which is very efficient but quite expensive. Since in my lab we used to buy 2 tubes of LIPOFECTAMINE every week , we would like to cut...
04 September 2013 6,547 4 View
I am trying to extract RNA from cells (to do a RT-PCR) and usually it works, but now it stopped working and the RNA is always degraded. I use a Trizol-based extraction. So far, I changed every...
04 January 2013 9,026 26 View
I successfully performed RT-PCR on different cell lines several times, but now it is not working anymore. Some cDNA are amplified while others are not. So, first I changed the enzymes (either for...
17 December 2012 2,566 29 View
