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Questions related from Daniel X Johansson
Dear community! Does anyone know how to create a solution of Zn2+ (at least 200mM, but even better at 1M) at pH 7, but avoiding Zn2+ from precipitation as Zn(OH)2? The solution will be used at...
12 January 2022 9,253 3 View
Does anyone know how the engineering of NEBs "High Fidelity" (HF) restriction enzymes work? I guess they are mutants of the original enzyme that have been selected via some clever library...
16 November 2017 9,597 0 View
We want to create a semi-random expression library (around 30aa). This could of course be done by using poly-N oligonucleotides, but there is a newer technology where the oligo is synthesized...
28 May 2014 5,325 2 View
The Pertussis Toxin is still active long after clearing bacteria. Is this because of already intoxicated cells or does a pool of toxin exist extracellularly that intoxicates new cells? My question...
12 February 2014 4,185 1 View
I'm looking for a protocol that allows isolation of plasmid DNA from E.coli but that is non-denaturing i.e. I want to keep any DNA binding proteins on the isolated plasmids. I want to get rid of...
21 January 2014 1,843 7 View
Longer Explanation: I would like to express a fusion of protein X and Y in prokaryotes. However, I want Y to be expressed by itself as well (from the same promoter). In a mammalian system (which I...
09 July 2013 1,901 4 View
