Daniel Chaves de Lima

5 Questions 9 Answers 0 Followers

Questions related from Daniel Chaves de Lima

How does YMB medium looks like after a few days of bacteria inoculation?

Hello, everyone I've recentely isolated bacteria from pigeon pea (Cajanus cajan) nodules on YMA medium and then transferred some colonies to YMB (Yeast Mannitol Broth) medium. This is the first...

02 May 2024 3,283 4 View

Does anyone know a tool for viral protein interaction prediction?

Hello everyone. I am looking in the internet for a tool in which I can predict viral proteins interactions with proteins from other organisms. At least until today, STRING and STICH do not have...

02 December 2017 270 1 View

How can I infer that two genes are paralogous using phylogenetic analysis?

Hello, everyone. I have two genes that are known to be paralogues (in bacteria) although I've never seen any work in the literature proving this assumption. I would like to conduct a phylogenetic...

06 May 2015 3,001 6 View

Does E. coli K-12 is suitable for expression of genes driven by T7 promoter?

Hello, scientists. I want to complement an E. coli mutant (derived from K-12) (http://www.ncbi.nlm.nih.gov/pubmed/16738554) with a gene that is cloned in a vector with a T7 promoter. I'd like to...

25 February 2015 9,642 14 View

Which plasmids should I use in a Complementation test?

Hello. I have a sodC (E. coli) mutant and I want to verify if a specific gene complements the sodC deletion. Which kind of plasmid should I use to clone this gene? It can be a plasmid with...

15 November 2014 1,063 3 View