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Questions related from Daniel Haines
A 10mM His buffer formulated at pH 6.2 (at 25*C) reads 6.4-6.5 when removed from a 2-8*C cooler. If you were to use this cold buffer to dilute a mAb which is currently in the same pH 6.2 buffer...
09 March 2018 2,768 3 View
We lyophilized 50mg/ml mAb with PI of 7.5 in a 10mM His buffer pH 6.2. Upon reconstitution the pH is 6.5. Does anyone have an explanation for this? I am under the impression His is much less...
12 December 2017 3,447 1 View
