Daniel Fritz

3 Questions 3 Answers 0 Followers

Questions related from Daniel Fritz

IHC strategy question: Can I combine a strepavidin-HRP "secondary" with an ABC kit to visualize protein?

I have a biotinylated monoclonal primary antibody that I wish to use to probe liver sections. I have a streptavidin-HRP that I can use as a "secondary" and a DAB substrate to visualize. Will...

06 September 2017 7,058 0 View

How to determine a good positive control for mouse bone marrow RT-PCR DNA gel?

I'm running a RT-PCR gel on cDNA derived from mouse bone marrow on two different genotyped mice. I'm trying to establish a good positive control that will show up in both mice. How can I find...

21 June 2016 4,758 4 View

Does anyone have a flow cytometry protocol using DCFDA?

I've been trying to adjust a protocol for ROS staining using DCFDA and am unsure of what to use for a single color control for compensating the cytometer. I've tried using stimulated cells...

19 February 2015 9,152 5 View