Daisy Jones

3 Questions 1 Answers 0 Followers

Questions related from Daisy Jones

How to segment the cytoplasm (excluding the nucleus) on imageJ?

Hi all, Does anybody know how to segment the cytoplasm of cells using imageJ but excluding the nucleus (creating a donut-type selection). I would like to use this to create an region of interest...

04 August 2021 894 3 View

What is the minimum volume of media you can use per well of a 96-well plate in HUVEC culture?

Hi all, Does anyone have experience working with small media volumes when treating HUVEC (or any cell type really) in a 96-well plate? For context, I will be collecting the media after a...

08 June 2021 3,290 1 View

Can the use of gelatin when plating HUVEC impact the result of cell lysate samples on an ELISA?

Hi everyone, I am interested to know if anyone has had any experience working with HUVEC cell lysates and running them on ELISA's? We seed our HUVEC on a gelatin matrix- could this impact our...

07 June 2021 8,504 4 View