Dacquin Kasumba

3 Questions 13 Answers 0 Followers

Questions related from Dacquin Kasumba

How long should we wait to see clear plaques when doing a plaque assay with IAV PR8?

I have been trying to perform plaque assay with PR8 but some times I am able to see clear plaques at 48h and sometimes there almost no plaques at 48h when I repeat the experiment. the protocol is...

17 April 2015 2,243 3 View

Trying to perform cloning using CRISPR/Cas9 kit but it seems as if the efficiency is very low with the kit or system. Has anyone experienced this?

CRISPR/Cas9 system is a system normally used by some microorganisms to repair DNA defects in their genome. This system is now used as an efficient tool for genome engineering. I am currently...

20 April 2014 9,941 5 View

What is the difference between blunt-ended dsRNA and dsRNA with overhangs (viral RNA)?

It might just be a question of semantics but I would appreciate if somebody could help clarify this. Is the difference (only) the fact that overhang dsRNAs have unpaired nucleotides at the end and...

20 January 2014 7,438 1 View