Cushla Mckinney

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Questions related from Cushla Mckinney

Tēna koutou, has anybody used the Neon electroporation system on NCI-N87 cells and, if so, what parameters did you use?

I am trying to CRISPR a number of gastric cell lines but can't find any published parameters for these particular cells and am hoping to avoid doing a full optimisation.

25 August 2022 4,239 0 View

Why are the SV40PolyA signal and downstream sequence elements inverted in many common expression plasmids?

Rather than have the SV40 PolyA sequence and 3' elements 5' to 3' on the coding strand, many reporeter expression vectors (such as EGFP) have the PolyA 5' to 3' on the opposite strand (with the...

21 July 2021 1,543 2 View

Any advice on efficient transfection of THP-1 cells?

Good morning, I am trying to introduce an expression plasmid into THP-1 cells and have come across a couple of papers that describe electroporation techniques but on different machine than we have...

19 November 2015 1,287 3 View