Craig McBeth

10 Questions 8 Answers 0 Followers

Questions related from Craig McBeth

Delamination of Au and Ag from Ti layer using gentle cleaning, why won't my layers adhere?

Hello all, I have been using a vapor deposition method (electron beam evaporation) to deposit a 10nm layer of Ti and a second layer of either Ag or Au at 250nm, on a sheet of ITO. They have great...

17 March 2020 1,032 2 View

Is it possible to fix cells stained with thioflavin T?

Hello all, I've recently been exploring the use of ThT as a membrane potential dye, I was hoping to ideally fix cells after I have treated the cells with ThT, however I have not found any...

31 May 2019 1,170 2 View

Reference electrodes - is electrolyte required?

Hey all, I have used a standard Ag/AgCl electrode for a while now but I've just moved institutions and a lab member is stating the electrolyte is not required for aqueous voltammetric analysis,...

23 January 2019 2,341 4 View

Why doesn't my cobalt phthalocyanine electrode look similar to that of previous literature?

Hello all, I have been trying to modify a plain printed carbon electrode with cobalt(II) phthalocyanine and I have found my CV does not look like any of the CVs in literature. I have attached a...

09 February 2018 6,383 3 View

Crosslinking polymer and mediator, printed electrode keeps 'cracking' and separating from substrate using glutaraldehyde, solutions?

Hello all, I've been trying for a while now to crosslink dopamine to chitosan using various means, more recently I have been using glutaraldehyde. My electrode is made in house using an ink from...

06 December 2017 4,211 1 View

Is it possible to disintegrate single wall carbon nanotubes?

I recently performed a two step purification of some single wall carbon nanotubes. First step involved refluxing 100mg of CNTs in 3M HCl for 2 days, which appeared to work effectively. They were...

20 July 2017 7,151 4 View

Is it possible to perform double solvent casting on a printed electrode?

Hello all, For the past week I have been working on a new electrode, the process however uses a double solvent cast in the construction of the electrode. Initially a layer of CNT + Nafion in...

16 February 2017 2,884 2 View

Looking for studies focusing on the assessment of culture media using electrochemical methods?

Hello all, I'm trying to find some literature on the electrochemical quantification of certain metabolites, amino acids or anything really, in cell culture media e.g. DMEM, EMEM, RPMI etc. Ideally...

27 April 2016 1,922 3 View

Griess reagent issues/ queries; no observable colouration, why?

Hello all, I've been trying to perform a Nitrite/ Griess assay on isolated cell media but no detectable traces of Nitrite is present. The media itself is phenol red-free MEM supplemented with...

13 May 2015 6,076 4 View

No colour is produced for samples or for the standard of my GSH assay, what could be missing?

I've been trying to perform a reduced GSH assay on some cell lysates and no colour is being produced upon the addition of DTNB (Ellmans). I have been adding 10uL of cell lysates to each well and...

30 April 2015 10,014 10 View