Cora Olpe

3 Questions 2 Answers 0 Followers

Questions related from Cora Olpe

Does anyone know of a good liquid DAB + chromogen kit?

I've been using the DAKO K346811-2 one but it's been discontinued. I then ordered the PierceTM one from thermo fisher (34002) but it's really rubbish. Does anyone know of a suitable alternative?...

08 August 2017 3,256 1 View

Has anyone ever stained fixed cells with fluorophore-conjugated antibodies and analysed by flow cytometry after 3 days?

Because of logistics I have to store my stained and fixed cells for 3 days before flow cytometry. Has this ever worked for anyone? I am used to staining and analysing on the same day...

05 May 2016 4,087 4 View

Has anyone ever done RT qPCR for MEKK1 in human cells and can provide me with primer sequences?

I am using gefitinib to inhibit the EGFR. I want to perform RT qPCR on EGFR target genes to make sure the drug is actually working.

02 February 2016 9,179 2 View