Colin Mann

4 Questions 2 Answers 0 Followers

Questions related from Colin Mann

Source of Native PAGE vs SDS PAGE weight discrepancies?

I am wondering if anyone can explain some apparent weight discrepancies between SDS-PAGE and Blue Native PAGE. I understand that the underlying principle is different in each, which may produce...

23 September 2019 993 2 View

Unknown bands pervasive in SDS-PAGE?

I am having problems with some samples in SDS-PAGE. For 2 specific proteins (one viral protein one serum protein) I am getting almost consistent unexpected bands across the gel. I have included...

06 August 2019 3,734 1 View

What are the mechanism of protection underlying passive immunization?

I am curious as to the particular mechanisms by which exogenous antibody can induce protection. Considering these antibodies do not result in natural immunity, do they have limited functions in...

10 July 2019 1,541 2 View

Does overexpression of proteins in vitro affect quality of the final product?

I have read some papers concerning expression levels of proteins and instances of misfolding to due to "back up" during cellular processing. I am expressing viral surface proteins and derived...

12 April 2019 5,496 8 View