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Questions related from Colin Davies
I am looking at undertaking AP-MS using click chemistry following the addition of a compound. I realise this can be used to look at compound-protein interactions, but would I expect to pulldown...
25 July 2022 3,475 1 View
Can I do a BP cloning reaction with a pDONR plasmid containing AttP sites and a pMx plasmid containing the AttB sites flanking my insert sequence, or does the BP reaction only work on linear PCR...
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Has anyone used trypan blue to look at cell viability in fibroblasts (HFF cells)? I find all cells seem to take up the dye making it difficult to decipher between live/dead cells.
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