Clinton A Oakley

6 Questions 7 Answers 0 Followers

Questions related from Clinton A Oakley

Clearing blocked nanoViper liquid chromatography tubing?

Does anyone have any tips on clearing blocked nanoViper (20 uM ID) tubing in a Dionex 3000 for LC-MS/MS? We're having recurring high pressure errors in our loading pump, and we've isolated it to a...

29 July 2020 3,962 3 View

Strategy for using STRING with non-model organisms?

I would like to use STRING for functional analysis of proteome data, as it appears to be quite powerful. Unfortunately I'm working with an organism that isn't in STRING's database, nor is there...

24 January 2019 3,181 3 View

Troubleshooting ESI LC MS/MS chromatograms--sample contamination?

We are having problems with an ESI LC MS/MS proteomics experiment that are difficult to pin down. Samples were prepared in batches over the course of a week, the first batches worked very well but...

13 July 2016 9,679 10 View

Why would the dark-adapted quantum yield of chlorophyll fluorescence be lower than that of the same algae culture under low light?

We are using a PAM fluorometer to measure the quantum yield of chlorophyll fluorescence in an intact microalga culture. When the culture is dark-adapted for 20-30 minutes, the quantum yield is...

23 February 2016 8,357 2 View

How to reduce high background noise in an LC MS/MS experiment?

We are attempting a shotgun proteomics experiment using LC ESI-MS/MS with an Orbitrap LTQ XL, but are suddenly encountering a problem with excessive background noise. The noise level is fairly...

24 February 2015 6,417 2 View

Is there a configuration file available for Mascot Distiller for metabolic 13C-labeling protein quantification without 15N labeling?

We are considering quantitative shotgun proteomics experiments on cultured marine algae using ESI-LC MS/MS followed by analysis by Mascot. It is not overly difficult to culture cells in a 99% 13C...

24 March 2014 9,181 1 View