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I used E64 to inhibit lysosome activity, could you suggest me a good positive control to check if the drug works appropriatly?
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I need to mix human neurons with mouse neurons or astrocytes. So I have to pass and count human neurons, but I can immage that is very dangerous and they tend to die. Let me know if somebody has...
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In particular I have astrocytes cells. Thanks in advance
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In particular I have some problems about aggregation at first step: the cells become "dark" after 2 hours. When I used the same protocol with mouse ESC I didn't have any problems.
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