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Any one have experienced to conjugate peptide with dye? I have protected peptide and i want to conjugate with dye which has NHS ester. I am confused because for conjugating the condition should be...
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I use LS 180 cell line that already trypsinized. I read in ATCC, LS 180 have never been trypsinized. so after that, I never use trypsin but my cells easily detach now. What should I do?
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