Chunlei Yuan

4 Questions 4 Answers 0 Followers

Questions related from Chunlei Yuan

How to raise yield when I extraction RNA from mice peritoneal macrophages?

I can only extraction about 1.5ug RNA from 3*10^6 mice peritoneal macrophages, which is much less than normal cells. How to raise yield?

17 November 2023 8,120 5 View

Will the cytosolic Ca2+ of myoblast elevated during the initiation of differentiation?

Someone said that transient Ca2+ depletion from the endoplasmic reticulum is critical for skeletal myoblast differentiation. I found that one gene deficiency in myoblast leads to precocious...

29 August 2023 2,895 0 View

How to measure myofiber cross-section area and fiber type proportion automatically?

Muscle frozen sections were stained with antibody against Laminin.

23 March 2023 282 2 View

Where is the inguinal white fat in mouse?

I want to dissect inguinal white fat in mouse, but I don't know where exactly it is. And I am confused whether posterior subcutaneous adipose tissue is the same with inguinal adipose tissue.

11 February 2022 6,709 9 View