Christoph Kreer

2 Questions 6 Answers 0 Followers

Questions related from Christoph Kreer

Why is my protein not re-dissolving after ammonium sulfate precipitation?

I am facing another problem during my protein purification. I purify a 120 kDa protein which is overexpressed in pichia pastoris. The protein is His-tagged (C-terminus) and FLAG-tagged...

07 July 2013 4,026 6 View

How to increase the binding of a His-tagged glycoprotein to Ni-NTA resin?

I try to purify a his-tagged protein which is either secreted and glycosylated or intracellularly expressed and not glycosylated (using Pichia pasotris). I am using the Ni-NTA resin from M&N. The...

05 May 2013 8,105 24 View