Christiana Lagudas

2 Questions 1 Answers 0 Followers

Questions related from Christiana Lagudas

Will adding DNAse to lysate in phosphate buffer affect its activity?

Hello, everybody. I am looking to add a DNAse treatment step to my bacterial lysis (gram-negative) procedure for crude enzyme extraction because my lysate always ends up being too viscous. We...

15 April 2024 149 2 View

Promega Passive Lysis Buffer for Bacterial Cells?

Are there any conditions where it would be possible to lyse gram-negative bacteria using the Promega PLB? I would need to lyse the cells to test for crude enzyme activity but I don’t have a...

31 January 2024 7,593 1 View