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I have been using the Euromedex BACTH system to test for protein interaction. After repeatedly selecting for my “prey” plasmid, I was unsuccessful dropping the “bait” plasmid. I decided to design...
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Please address the best way to drop a plasmid. Background: I have a "bait" plasmid resistant to kanamycin and a "prey" plasmid resistant to carbenicillin. After many rounds of streaking on...
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