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Questions related from Christian Baumgartner
We want to do some FACS analysis on freshly isolated primary mouse hepatocytes. So far, we had problems with high autofluorescence. Any suggestions to solve this problem? Can the isolation method...
30 January 2014 4,986 9 View
I am planning to use 5-FU injections into my mice. I have to inject 150 mg/KG body weight - since the solubility in PBS or physiological NaCl is quite low people are also preparing solutions with...
14 November 2013 1,656 1 View
Does anybody have experience in this field. I am actually working on a KO model with increased AKT activation in the hematopoietic system especially upon stress situations. I wanted to test...
16 October 2013 6,282 6 View
I would like to characterize my hematopoietic progenitors using CD16/32-PE vs CD34 APC. CD16/32 is the FcyRII/III to characterize CMPs, GMPs and MEPs in mouse bone marrow. Usually, I use...
06 September 2013 5,024 1 View
I sorted bm LSK cells and got around 15,000 cells from 4 isolated bones (femur + tibia). We are interested in LSK CD150+ CD48- subfractions as well as CMPs, GMPs, MEPs, afterwards we want to...
04 September 2013 5,967 8 View
I want to study intracellular signaling in mouse hematopoietic stem/progenitor cells. Due to the fact that stem/progenitor cells are a very small population, it would be cool to use phosflow...
03 September 2013 8,854 3 View
